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《环境科学与工程前沿(英文)》 2022年 第16卷 第2期 doi: 10.1007/s11783-021-1458-7
•Tryptophan protein, and aromatic protein I/II were the key identified proteins.
关键词: Sludge pretreatments Dissolved organic nitrogen Proteins Amino acids Structural equation model Metagenomic sequencing analysis.
Metagenomic analysis on resistance genes in water and microplastics from a mariculture system
《环境科学与工程前沿(英文)》 2022年 第16卷 第1期 页码 4-4 doi: 10.1007/s11783-021-1438-y
Microplastics existing widely in different matrices have been regarded as a reservoir for emerging contaminants. Mariculture systems have been observed to host microplastics and antibiotic resistance genes (ARGs). However, more information on proliferation of ARGs and metal resistance genes (MRGs) in mariculture system at the presence of microplastics is needed.
关键词: Antibiotic resistance genes Metal resistance genes Metagenomic analysis Microplastics Mariculture
Plastic materials and water sources actively select and shape wastewater plastispheres over time
《环境科学与工程前沿(英文)》 2022年 第16卷 第11期 doi: 10.1007/s11783-022-1580-1
● Wastewater MPs exhibited resistomes and therefore health threats.
关键词: Microplastics Whole-genome metagenomic sequencing Plastisphere Plastic degradation MHETase
Quan Zheng, Minglu Zhang, Tingting Zhang, Xinhui Li, Minghan Zhu, Xiaohui Wang
《环境科学与工程前沿(英文)》 2020年 第14卷 第4期 doi: 10.1007/s11783-020-1239-8
关键词: Chromium nanoparticles (Cr NPs) Metagenomic analysis Metatranscriptomic analysis Molecular ecological networks (MENs) Nitrogen and phosphorus removal
《环境科学与工程前沿(英文)》 2023年 第17卷 第1期 doi: 10.1007/s11783-023-1602-7
● Four Ca. Brocadia species were observed during the spontaneously enrichment.
关键词: Anammox Candidatus Brocadia Functional potential Cooccurring mechanisms Swine wastewater treatment facilities
Comparative analysis of impact of human occupancy on indoor microbiomes
《环境科学与工程前沿(英文)》 2021年 第15卷 第5期 doi: 10.1007/s11783-020-1383-1
• Exposure to indoor microbiomes is a public health concern in educational facilities.
关键词: Built environment Indoor microbiome Occupant Building Sequencing
《医学前沿(英文)》 页码 889-906 doi: 10.1007/s11684-023-0994-x
关键词: primary central nervous system lymphoma whole-genome sequencing TMSB4X copy number variation gene mutation
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《医学前沿(英文)》 2013年 第7卷 第3期 页码 280-289 doi: 10.1007/s11684-013-0265-3
Many gene fusions have been recognized as important diagnostic and/or prognostic markers in human malignancies. In recent years, novel gene fusions have been identified in cases without prior knowledge of the genetic background. Accompanied by a powerful computational data analysis method, new genome-wide screening approaches were used to detect cryptic genomic aberrations. This review focused on advanced genome-wide screening approaches in fusion gene identification, such as microarray-based approaches, next-generation sequencing, and NanoString nCounter gene expression system. The fundamental rationale and strategy for fusion gene identification using each biotech platform are also discussed.
关键词: gene fusion cancer microarray next-generation sequencing NanoString nCounter system
Application of machine learning technique for predicting and evaluating chloride ingress in concrete
Van Quan TRAN; Van Loi GIAP; Dinh Phien VU; Riya Catherine GEORGE; Lanh Si HO
《结构与土木工程前沿(英文)》 2022年 第16卷 第9期 页码 1153-1169 doi: 10.1007/s11709-022-0830-4
关键词: gradient boosting random forest chloride content concrete sensitivity analysis.
Chen Qian, Wei Chen, Wei-Hua Li, Han-Qing Yu
《环境科学与工程前沿(英文)》 2017年 第11卷 第4期 doi: 10.1007/s11783-017-0962-2
关键词: Wastewater treatment plants (WWTPs) Excitation-emission matrix (EEM) Parallel factor (PARAFAC) Parallel factor framework-clustering analysis (PFFCA)
Exome sequencing greatly expedites the progressive research of Mendelian diseases
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《医学前沿(英文)》 2014年 第8卷 第1期 页码 42-57 doi: 10.1007/s11684-014-0303-9
The advent of whole-exome sequencing (WES) has facilitated the discovery of rare structure and functional genetic variants. Combining exome sequencing with linkage studies is one of the most efficient strategies in searching disease genes for Mendelian diseases. WES has achieved great success in the past three years for Mendelian disease genetics and has identified over 150 new Mendelian disease genes. We illustrate the workflow of exome capture and sequencing to highlight the advantages of WES. We also indicate the progress and limitations of WES that can potentially result in failure to identify disease-causing mutations in part of patients. With an affordable cost, WES is expected to become the most commonly used tool for Mendelian disease gene identification. The variants detected cumulatively from previous WES studies will be widely used in future clinical services.
关键词: genetics whole-exome sequencing Mendelian disease disease gene
《医学前沿(英文)》 2022年 第16卷 第2期 页码 240-250 doi: 10.1007/s11684-021-0909-7
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《医学前沿(英文)》 2015年 第9卷 第3期 页码 322-330 doi: 10.1007/s11684-015-0408-9
This study systematically evaluates the TCGA whole-transcriptome sequencing data of hepatocellular carcinoma (HCC) by comparing the global gene expression profiles between tumors and their corresponding non-tumorous liver tissue. Based on the differential gene expression analysis, we identified a number of novel dysregulated genes, in addition to those previously reported. Top-listing upregulated (CENPF and FOXM1) and downregulated (CLEC4G, CRHBP, and CLEC1B) genes were successfully validated using qPCR on our cohort of 65 pairs of human HCCs. Further examination for the mechanistic overview by subjecting significantly upregulated and downregulated genes to gene set enrichment analysis showed that different cellular pathways were involved. This study provides useful information on the transcriptomic landscape and molecular mechanism of hepatocarcinogenesis for development of new biomarkers and further in-depth characterization.
Exploring the cancer genome in the era of next-generation sequencing
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《医学前沿(英文)》 2012年 第6卷 第1期 页码 48-55 doi: 10.1007/s11684-012-0182-x
The emergence of next-generation sequencing technologies has led to dramatic advances in cancer genome studies. The increased efficiency and resolution of next-generation sequencing greatly facilitate the detection of genetic, genomic, and epigenomic alterations, such as single nucleotide mutations, small insertions and deletions, chromosomal rearrangements, copy number variations, and DNA methylation. Comprehensive analysis of cancer genomes through approaches of whole genome, exome, and transcriptome sequencing has significantly improved the understanding of cancer biology, diagnosis, and therapy. The present study briefly reviews the recent pioneering studies on cancer genome sequencing and provides an unprecedented insight into the landscape of genomic alterations in human sporadic cancers.
关键词: next-generation sequencing cancer genome whole genome sequencing exome transcriptome
Xiaolin Sheng, Rui Liu, Xiaoyan Song, Lujun Chen, Kawagishi Tomoki
《环境科学与工程前沿(英文)》 2017年 第11卷 第3期 doi: 10.1007/s11783-017-0929-3
关键词: Digested piggery wastewater Intermittent aeration Microbial community Partial nitrification–denitrification process Sequencing batch reactor (SBR)
标题 作者 时间 类型 操作
Anaerobic digestion of sludge by different pretreatments: Changes of amino acids and microbial community
期刊论文
Plastic materials and water sources actively select and shape wastewater plastispheres over time
期刊论文
Insights from metagenomic, metatranscriptomic, and molecular ecological network analyses into the effects
Quan Zheng, Minglu Zhang, Tingting Zhang, Xinhui Li, Minghan Zhu, Xiaohui Wang
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Genome-resolved metagenomic analysis reveals different functional potentials of multiple Brocadia species
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Analysis of the genomic landscape of primary central nervous system lymphoma using whole-genome sequencing
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Identification of cancer gene fusions based on advanced analysis of the human genome or transcriptome
null
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Application of machine learning technique for predicting and evaluating chloride ingress in concrete
Van Quan TRAN; Van Loi GIAP; Dinh Phien VU; Riya Catherine GEORGE; Lanh Si HO
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A chemometric analysis on the fluorescent dissolved organic matter in a full-scale sequencing batch reactor
Chen Qian, Wei Chen, Wei-Hua Li, Han-Qing Yu
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Accurate quantification of 3′-terminal 2′-O-methylated small RNAs by utilizing oxidative deep sequencing
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TCGA whole-transcriptome sequencing data reveals significantly dysregulated genes and signaling pathways
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